X-CGD mice exhibited enhanced response to sterile tissue injury. (A) Total WBC counts and (B) ANCs was determined in peripheral blood of WT and X-CGD mice 4 and 8 hours postperiodate injections (n > 9 in each group from ≥3 separate experiments). (C) Peritoneal neutrophils (Ly6G⁺, Ly6C^int) and (D) peritoneal monocytes (Ly6G⁻, Ly6C^hi/int) were identified by flow cytometry in mice sacrificed 2 hours, 4 hours, 8 hours, or 24 hours postperiodate challenge. Data are presented as mean ± standard deviation and P values for each time point were determined using the Student t test. *P < .05, **P < .1, ***P < .001. Other mice were challenged i.p. with MSU or NC. (E) Peritoneal neutrophils and (F) monocytes were enumerated after 4 hours. Combined total of n = 6 mice in each group from 2 separate experiments. Data are presented as mean ± standard deviation and statistical differences calculated at each time point using the Student t test. *P < .05, **P < .01, ***P < .001. NC, necrotic cell lysate; WBC, white blood cell.