NADPH oxidase deficiency augmented IL-1 response. (A-C) Sorted resident peritoneal macrophages, BMDMs, and BMDCs from WT (▪) or X-CGD mice (□) were primed in vitro with ultrapure LPS prior to stimulation with necrotic EL4 lysates or inflammasome agonists, as indicated. IL-1α and IL-1β levels were determined by ELISA in cell-free supernatants. *P < .05, **P < .1, ***P < .001. Representative data from 1 of 3 experiments. For each experiment, 1 WT and 1 X-CGD mouse were used to isolate ResM or generate BMDMs and BMDCs. (D-E) Lethally irradiated recipient mice were transplanted with donor marrow to generate the following groups (donor into recipient): WT into WT, X-CGD into X-CGD, X-CGD into WT, and WT into X-CGD. Eight weeks after transplant, mice were challenged with periodate i.p. and peritoneal inflammation assessed 4 hours later by enumerating (D) total peritoneal WBCs and (E) neutrophils as determined by flow cytometry. Mean ± standard deviation is shown for 1 of 3 representative experiments with n = 3 per group. Differences for each cytokine (IL-1α or IL-1β) between genotypes were analyzed for every cell type and experimental condition separately and P values determined by the Student t test. *P < .05, **P < .01, ***P < .001. NC, necrotic cell lysate; ResM, sorted resident peritoneal macrophage.