Antibodies to G-CSF or Ly6G prevented neutrophil mobilization and subsequent recruitment to peritoneal cavity. (A) Serum levels of G-CSF were estimated by ELISA 4 hours postperiodate injections (n > 5). Data are presented as mean ± standard deviation was analyzed using the Mann-Whitney U test; *P < .05. (C-G) Mice were injected IV with neutralizing antibodies to mouse G-CSF or with anti-Ly6G to deplete neutrophils, then challenged with periodate and sacrificed after 8 hours. (B,E) WBC and (C,F) ANC in peripheral blood were determined by Hemavet analysis, and (D,G) peritoneal neutrophils enumerated by flow cytometry for anti-G-CSF–injected mice and by cytospins for anti-Ly6G–injected mice. For G-CSF antibody, n = 5 per group from 1 experiment; anti-Ly6G, n = 6 per group from 2 separate experiments. For B-G, data are presented as mean ± standard deviation and P values determined using 1-way ANOVA with Tukey correction. . *P < .05, **P < .01, ****P < .0001.