Effects of everolimus on mTOR signaling in TCL cell lines. (A-B) ALCL and CTCL cell lines were treated with 1 and 10 nM of everolimus overnight. Whole cell lysate were then subjected to western blotting with phospho-specific antibodies to mTOR, S6, 4EBP1, and AKT as indicated. (C) Karpas 299, SR786, HuT 78, and MyLa TCL cell lines were treated with everolimus overnight and western blotting was performed using phosho-eIF4E antibody.