Impaired differentiation in macrophages from Nbs1^∆B/∆B mice. Macrophages were differentiated from the BM of WT and Nbs1^∆B/∆B mice, and the surface expression of differentiation markers was determined by using antibodies and flow cytometry. (A) After 6 days of differentiation, cells were stained. (B) Time course of differentiation of Ly6C^highCD11b⁺ and F4/80⁺CD11b⁺ cells. Each experiment was performed in triplicate and the results are shown as mean ± SD. All assays are representative of at least 4 independent experiments showing similar results. *P < .05, **P < .01, and ***P < .001 in relation to the controls when all the independent experiments had been compared.