Exacerbated inflammation in Nbs1^∆B/∆B mice treated with DNFB. (A) A representative sample of histologic images of DNFB-treated ears (at 7 day) is shown. At the right, the net weight of ears treated for 7 days with DNFB (8 mice) is shown. This was calculated by subtracting the weight of the DNFB-treated right ear from that of the acetone-treated left ear that was used as control. The ear thickness measurements were taken from the histologic images using Fiji software. Only DNFB-treated ear thickness is shown. AU, arbitrary unit. (B) Ear sample-derived mRNA was used to determine the expression of proinflammatory and anti-inflammatory markers by qPCR. The results are shown as individual values and as the mean of the 8 WT and 8 Nbs1^∆B/∆B mice. *P < .05 and **P < .01 in relation to the corresponding controls.