Development of a conditional mouse Cdh5 knockout. (A) Breeding strategy to develop Cdh5^flox/flox: Scl-Cre-ER^T mouse. (B) PCR-based strategy to genotype WT, Cdh5^flox/flox, Cdh5^flox/+:Scl-Cre-ER^T, Cdh5^flox/flox: Scl-Cre-ER^T, and Scl-Cre-ER^T using primers listed in supplemental Table 4. (C) Schema to delete Cdh5 in Scl⁺ cells in Cdh5^flox/flox:Scl-Cre-ER^T. Tamoxifen was injected in pregnant dam from E5.5 to E10.5 and AGM were harvested on E11.5 for CFU analyses. (D) Average number of GEMM colonies per 1 e.e. AGM derived from Cdh5 ^flox/flox (n = 10), Cdh5 ^flox/+:Scl-Cre-ER^T (n = 7), and Cdh5 ^flox/flox:Scl-Cre-ER^T (n = 11), respectively. (E) Percentage Cdh5 alleles in colonies derived from Cdh5 ^flox/+:Scl-Cre-ER^T (n = 55 were either fl/+ or ∆/+), and Cdh5 ^flox/flox:Scl-Cre-ER^T (n = 72 were fl/fl, ∆/+, or ∆/∆). t test; error bars indicate s.e.m.