Figure 3
![Figure 3. GO-203 increases hydrogen peroxide (H2O2) levels in CTCL cells, which is abrogated by addition of N acetyl cysteine (NAC). (A-D) HuT-78 and H9 cells were left untreated and treated with 5 μM GO-203 or CP-2 each day for 3 days. (C and D) The GO-203-treated cells were also incubated in the presence of 5 mM NAC for the last 2 days. Cells were incubated with c-H2DCFDA for 30 minutes. Fluorescence of oxidized DCF was measured by flow cytometry (left). The results are expressed as the relative hydrogen peroxide level (mean ± standard deviation [SD] of 3 determinations) compared with that obtained for control cells (right).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/126/3/10.1182_blood-2015-02-628149/4/354f3.jpeg?Expires=1735535393&Signature=M0ccL6DAge8GPQxme4qGlq06R3bFEXroDdCjJzJUM1TsiwwvNybmtd8xX37EzWBSqHqu-nuirVac4QcUm0FEPd4XIdXrIqtGA7PB-in~KUolfVL~L80bi6v3ADXWA0zlIvRvxP-E6uZYb0LrJZE~VWQOe1oY8x6-xNTlDH24RCu-gEWyP7LFQ9W5U7Ni7mI0xiyJZvb3yLc6RLeb-Bdz-K~DpMoBtPlgLwLNdw0rFrCDWBULO91R~kgjTCmsDRFZSKA6ZjmuLME-k9wVBim9SWoDRx06-ABtHosGfVyDQ8hKuBs~RlGsl3fSrjkQBVrgqeAZ0OUgEK-rOVbcCMiV0A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
GO-203 increases hydrogen peroxide (H2O2) levels in CTCL cells, which is abrogated by addition of N acetyl cysteine (NAC). (A-D) HuT-78 and H9 cells were left untreated and treated with 5 μM GO-203 or CP-2 each day for 3 days. (C and D) The GO-203-treated cells were also incubated in the presence of 5 mM NAC for the last 2 days. Cells were incubated with c-H2DCFDA for 30 minutes. Fluorescence of oxidized DCF was measured by flow cytometry (left). The results are expressed as the relative hydrogen peroxide level (mean ± standard deviation [SD] of 3 determinations) compared with that obtained for control cells (right).
