Terminal differentiation and proliferation, but not specification, are defective in RPL11 deficiency. Erythroid cells at various stages of development were analyzed. (A-B) In situ hybridization for gata1 expression. (A) Four somites stage. (B) 30 hpf. (C) Time course of morphants in LCR2:EGFP transgenic zebrafish. (D-E) Flow cytometry analysis of EGFP-positive cells. (D) EGFP intensity. (E) Cell size, measured by forward scatter; *P < .05. (F) Wright-Giemsa staining of isolated erythroid cells collected from zebrafish embryos. (G) In situ hybridization for PCNA in uninjected or morpholino-injected embryos. Observed phenotype (G): uninjected = 24/24 morphants = 23/25.