Cord blood TILs show a significant CD8⁺ T-cell bias and higher CD8:T-reg ratio than peripheral blood TILs. Representative flow cytometry plots of (A) CD4⁺ and CD8⁺ T cells and (B) CD4⁺CD25⁺Foxp3⁺ T cells at injection and in TILs on day +10 and day +20 are shown. (C) CB TILs had a significantly lower CD4:CD8 ratio and a significantly higher CD8:CD4⁺ T-regulatory ratio compared with PB TILs on day +10 and day +20 after T-cell injection. The ratios are plotted on a log-scale as median and interquartile range.