Figure 2
![Figure 2. αDR3-treated T cells are less proliferative and maintain higher Treg frequency. T cells from αDR3 or isotype mAb–treated C57BL/6 mice were plated at 1:1 ratio to γ-irradiated Balb/c splenocytes in an MLR. (A) [3H] Thymidine incorporation of allogeneic responder cells (**P < .01, ***P < .001, ****P < .0001). (B) T cells were stained for FoxP3 before and 96 hours after MLR to assess Tregs (%) of CD4+ T cells (***P < .001). (C-E) Frequencies of TNFα (C), granzyme B (D), and T-bet (E) in CD8+ T cells are shown (*P < .05, ***P < .001). The results of more than 2 independent experiments are shown (N = 4, in total).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/126/4/10.1182_blood-2015-04-637587/4/546f2.jpeg?Expires=1735526255&Signature=ZmuJXnblZhJmVKgszpQocI~tSlFEV9KPe~lCtOtEZ27p8e6Sh3vgf0r95dPqC1Ml0swvy7yA7WjLwqqEuN8Vu-7roNeeIOOO530Pd3oB1ja8dr1il2esGephni90oRGjISi6fLecwwelLYwtarmkQwTVaywHPMpRAqHyzA6YYhsE4UJn4qUaWqYDa6Wu~f6UfkBdmxtaCUiGB8LdcFTvMS~JmmCbWbf3YzJsp16NM0MglmK2MQDVTP7wCxilYbLg0TisdmGxedQ734A4PRpJNglA1Jaf-Hofugtll4DHQyrA3G36Y8Npww54NY9Pzml95jR9KiyiHbK7LCCgt3vX4A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
αDR3-treated T cells are less proliferative and maintain higher Treg frequency. T cells from αDR3 or isotype mAb–treated C57BL/6 mice were plated at 1:1 ratio to γ-irradiated Balb/c splenocytes in an MLR. (A) [3H] Thymidine incorporation of allogeneic responder cells (**P < .01, ***P < .001, ****P < .0001). (B) T cells were stained for FoxP3 before and 96 hours after MLR to assess Tregs (%) of CD4+ T cells (***P < .001). (C-E) Frequencies of TNFα (C), granzyme B (D), and T-bet (E) in CD8+ T cells are shown (*P < .05, ***P < .001). The results of more than 2 independent experiments are shown (N = 4, in total).
