Donor treatment with αDR3 reduces GVHD mortality and alloreactive T-cell expansion after BMT. T cells were isolated from luc+ CD45.1+ C57BL/6 donors that were treated with αDR3 vs isotype mAb. Wild-type Balb/c recipients were lethally irradiated and transplanted with 1 × 106 T cells and 5 × 106 TCD-BM cells. Survival (A), GVHD score (B), and the relative weights (C) of recipient mice were assessed. Results are combined from 3 independent experiments. Survival (A) curves were compared by the log-rank test (****P < .0001). GVHD scores (B) and the relative weights (C) were compared by multiple Student t tests with Holm-Sidak correction (**P < .01, ***P < .001, ****P < .0001). (D) The bioluminescence signal from donor T cells on days 3, 6, 12, 20, 30, and 44 after BMT are analyzed. The total photon flux from either group (αDR3 vs isotype mAb–treated T cells) are compared by multiple Student t tests with Holm-Sidak correction (****P < .0001). The data were derived from 3 independent experiments. (E) Representative images of recipient mice on days 6 and 30 are shown.
