Platelet-derived FVIII. The 2bF8 lentiviral vector (LV) was constructed by transfection of lentivirus in 293T cells by plasmids containing hBDD-FVIII complementary DNA (cDNA). HSCs collected from the bone marrow of tibia and femurs of FVIIInull mice were transduced with 2bF8 LV. Platelets were isolated from HSCs in the presence of cytokines, and 2bF8 LV was detected in platelet α-granules. Following conditioning by a lethal dose of 1100 cGy total body irradiation, 6- to 8-week FVIIInull mice received 2bF8-transduced platelets by retro-orbital or tail-vein injection. Platelet FVIII expression was measured by chromogenic assay and anti-FVIII by Bethesda assay. FVIII function was assessed by survival after tail clip.7-9 BM, bone marrow.

Platelet-derived FVIII. The 2bF8 lentiviral vector (LV) was constructed by transfection of lentivirus in 293T cells by plasmids containing hBDD-FVIII complementary DNA (cDNA). HSCs collected from the bone marrow of tibia and femurs of FVIIInull mice were transduced with 2bF8 LV. Platelets were isolated from HSCs in the presence of cytokines, and 2bF8 LV was detected in platelet α-granules. Following conditioning by a lethal dose of 1100 cGy total body irradiation, 6- to 8-week FVIIInull mice received 2bF8-transduced platelets by retro-orbital or tail-vein injection. Platelet FVIII expression was measured by chromogenic assay and anti-FVIII by Bethesda assay. FVIII function was assessed by survival after tail clip.7-9  BM, bone marrow.

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