Administration of H2O, but not TNF-α or hemin, increases plasma Hb levels in C57BL/6 mice. Plasma Hb concentration in C57BL/6 or chimeric SCD mice (A) at baseline or 15 minutes after administration of saline (150 μL IV; n = 5 to 9 mice), (B) at 15 minutes after administration of saline or 15 and 60 minutes after administration of H2O (150 µL IV; n = 4 to 9 mice), (C) at 180 minutes after administration of TNF-α or vehicle (0.5 µg IP; n = 4 to 6 mice), and (D) at 120 minutes after administration of hemin or vehicle (40 µmol/kg IP; n = 3 to 6 mice). Plasma Hb is expressed as µM heme associated with Hb, assuming that Hb is tetramic. **P < .01 compared with basal C57BL/6; #P < .05, ##P < .01, and ###P < .001 compared with C57BL/6 plus saline; ●●P < .01 compared with vehicle (C57BL/6).
