Bioluminescence imaging of myeloperoxidase activity in C57BL/6 and chimeric SCD mice after administration of TNF-α and H2O. Quantification of systemic inflammation in (A) C57BL/6 and (C) chimeric SCD mice after treatment with TNF-α (0.5 µg IP; 180 minutes; n = 3 to 5 mice) or vehicle and H2O (150 µL IV; 15 or 60 minutes; n = 5 to 12 mice) or saline. *P < .05 and ***P < .001 compared with vehicle and ###P < .001 compared with saline. Illustrative images from each treatment in (B) C57BL/6 and (D) chimeric SCD mice. Mice were injected with a chemiluminescent probe that reacts with myeloperoxidase produced by activated phagocytes and/or neutrophils. Signal intensity was quantified as the photon flux (photons per second) within the region of interest.
