NO scavenging induces leukocyte recruitment, and NO donation reverses the systemic inflammation and leukocyte recruitment induced by H2O in C57BL/6 mice. (A) Plasma nitrate (NO3–) concentration in C57BL/6 mice at 2 to 30 minutes after administration of H2O (150 µL IV) or saline; n = 3 mice. WBC (B) rolling and (C) adhesion determined 15 minutes after administration of saline, H2O (150 µL IV), or PTIO (1.0 mg/kg IV); n = 34 to 48 venules from 4 to 6 mice. (D) Plasma Hb levels in C57BL/6 mice 15 minutes after receiving saline (150 µL IV), H2O (150 µL IV), PTIO (1.0 mg/kg IV), or DEANO (0.4 mg/kg IV) coadministered with either saline or H2O (150 µL IV); n = 3 to 11 mice. (E) Inflammatory status was analyzed by IVIS at 15 minutes after administration of H2O or saline (150 µL IV) in the presence or absence of DEANO (0.4 mg/kg IV; n = 3 to 6 mice). WBC (F) rolling, (G) adhesion, and (H) extravasation determined 15 minutes after coadministration of DEANO or vehicle (0.4 mg/kg IV) plus saline or H2O (150 µL IV) (n = 24 to 28 venules from 3 mice). *P < .05, **P < .01, and ***P < .001 compared with saline; ∞∞P < .01 compared with saline + DEANO; ●●P < .01 compared with H2O alone; #P < .05 and ###P < .001 compared with H2O + vehicle.
