Induction of HTLV-1 antigen-specific T cells by rVV in mice. (A) Mice (n = 3) in each group received a single rVV vaccination. Whole splenocytes were pooled and IFN-γ production was measured after stimulation (stimu) with overlapping peptides. (B) Mice were vaccinated with the rVV as shown. IFN-γ–producing cells among CD4- or CD8-depleted splenocytes were measured after stimulation with the pooled peptides (P1-P4). Data are presented as total number of spots from 4 measured pools. The bars represent median values. (C) The proportion of T cells isolated from rVV Tax M22- or HBZ LL/AA-vaccinated mice reacting to each peptide pool. The results shown are the average for each pool. (D) CD8⁺ T cells from vaccinated mice were stimulated as indicated, and cells positive for IFN-γ, CD107a, TNF-α, or IL-2 were measured. Cells stimulated with nonpulsed EL4 cells were used as a reference. The bars represent the mean ± standard deviation (SD) for all mice. At least 2 independent experiments were performed. *P < .05 by Student t test. APC, antigen-presenting cell.