Establishment of an HBZ-induced T-cell line. (A) Expression of various markers in the Ht48 cell line. Cells isolated from the lymph nodes (LN) of an HBZ-Tg mouse were injected intraperitoneally into immunodeficient (NOG and SCID) and immunocompetent mice (C57BL/6) as indicated. Eventually, splenocytes from a C57BL/6 mouse established the cell line we named Ht48. In vivo passage of this cell line was done by serial transplantation. (B) HBZ messenger RNA (mRNA) level in the Ht48 cell line. (C) Intraperitoneal inoculation of Ht48 into C57BL/6 mice decreased their survival in a dose-dependent manner. Survival curves were analyzed by the Kaplan-Meier method, and statistical difference was calculated by log-rank test. *P < .05 by log-rank test. (D) Ht48 inoculation (105 cells) via tail vein resulted in severe accumulation of cells in the liver and spleen after 4 weeks of inoculation. (E) Hematoxylin and eosin staining of tissues from the Ht48-inoculated mice. The data shown represent the mean ± SD of triplicate experiments. At least 2 independent experiments were performed. p.i., post inoculation.
