Successful BM reconstitution from Porcn^Del HSCs. (A) Rosa-CreER^T2/Porcn^Del BM cells formed hematopoietic progenitor colonies in culture. Porcn^Del (n = 5 wells, 2 mice) were compared with Porcn^WT donors (n = 4 wells, 2 mice). A mild increase in granulopoiesis was observed. Total: all types of colonies including granulocyte, erythrocyte, monocyte, megakaryocyte (GEMM), granulocyte (Gr), monocyte (M), granulocyte and monocyte (Gr/M) (*P < .05 and **P < .01. NS: P > .05, Mann-Whitney test). (B) Rosa-CreER^T2/Porcn^Del BM successfully reconstitutes sublethally irradiated recipient mice. Analysis was performed 5 months after BMT. Representative gating for donor BM lymphocytes and neutrophils (CD45.2⁺) (n = 4 and 5 for Porcn^WT and Porcn^Del mice, respectively, 2 independent experiments). (C) Quantification of donor neutrophil frequency in total BM cells from recipient mice. (D) Quantification of donor lymphocyte frequency in total BM cells from recipient mice. (E) Representative LT-HSCs, and HPC gating based on donor cells (CD45.2⁺) (right panel). Quantification of donor LT-HSCs, and HPC frequency in total BM cells from recipient mice (n = 4 and 5 for Porcn^WT and Porcn^Del mice, respectively, 2 independent experiments). (F) Representative myeloid progenitor gating based on donor cells (left panel). Quantification of donor myeloid progenitor frequency in total BM cells from recipient mice.