Inhibition of Wnt secretion by Porcn inhibitor in BM niche had limited effect on hematopoiesis. (A) Expression of Wnt target gene Axin2 was significantly downregulated in intestine samples of mice treated with 50 mg/kg for 20 days (2 independent experiments, P = .007, Mann-Whitney test). However, expression of Axin2, c-myc, and Cyclin D1 was not significantly altered in BM samples of these C59-treated mice. (n = 7; 2 independent experiments, P > .05, Mann-Whitney test). (B) The myeloid progenitor cells (1 leg) were not different in vehicle and C59-treated mice (n = 7; 3 independent experiments, P > .05, Mann-Whitney test). (C) C59 treatment did not alter the total LT-HSC, ST-HSC, and HPC numbers (1 leg). Representative flow cytometer data are shown in the left. The HSC numbers were graphed in the right. (n = 7; 3 independent experiments, P > .05, Mann-Whitney test). (D) The CLP cell numbers (1 leg) were not significantly different in vehicle and C59-treated mice (n = 7; 2 independent experiments, P > .05, Mann-Whitney test).