Proposed mechanism of FeCl₃-induced thrombosis. (A) Aggregates of washed RBCs, washed platelets, PPP, PRP, and whole blood (WB) form in microfluidic channels at all concentrations ranging from 50 mM to 1 M. Time (in seconds) to initial adherence of blood components and stable aggregate formation (unchanging or complete occlusion) is shown, where complete channel occlusion is denoted by cross-hatched bars. (B) Representative images of blood component aggregates in nonendothelialized microfluidics are shown at 50 mM. The presence of sheet-like protein aggregates is clearly visible in PPP. All scale bars represent 50 μm; N = 5.