Inhibition of G9a results in upregulation of γ-globin and downregulation of β-globin genes expression. RT-qPCR analysis of (A) γ-globin, (B) β-globin, and (C) α-globin gene expression in control and phase 2–treated cells at culture day 14. (D) RNA PolII and (E) H3K36me3 occupancy at γ- and β-globin genes in control and phase 2–treated cells at culture day 14. Error bars indicate SEM; n = 3 independent donors for each condition. Student t test values of *P < .05 or **P < .01 compared with control. cDNA, complementary DNA; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IgG, immunoglobulin G.