G9a methyltransferase activity is required for H3K9me2 and G9a distribution in the β-globin locus. (A) H3K9me2, (B) H3K27me2, and (C) G9a occupancy determined by ChIP at LCR HS sites and γ-, δ-, and β-globin gene promoters in control and phase 2–treated cells at culture day 14. Error bars indicate SEM; n = 3 independent donors for each condition. Values are compared with the value for control. *P < .05, **P < .01 by Student t test.