H3K9me2 protects γ-globin gene from looping with LCR. 3C assay measuring locus-wide crosslinking frequencies in control and phase 2–treated cells at culture day 14. The EcoRI fragments containing HSs of the LCR (orange bar) was used as the anchor region. The X-axis shows genomic coordinates and globin gene locations. EcoRI restriction sites are depicted by yellow triangles. Error bars indicate SEM; n = 3 independent donors for each condition.