B-cell developmental defect displayed in Relb^−/−cRel^−/− mouse is B-cell intrinsic. (A and C) Schematic for the generation of CD45.1 wild-type and CD45.2 Relb^−/−cRel^−/− mixed bone marrow chimeras. Bone marrow stem cells from CD45.1 wild-type and CD45.2 Relb^−/−cRel^−/− are mixed in a 50:50 ratio and then injected into a lethally irradiated CD45.1.2 wild-type or Relb^−/−cRel^−/− mouse. (B and D) Graphical plots of percentages of specific subsets relative to total B cells in indicated mixed bone marrow chimeras, as described previously. The gating strategy used in the analysis is depicted in Figure 3. Representative FACS plots see supplemental Figure 3. For panel B, n = 5; for panel D, n = 2. *P < .05; **P < .005; ***P < .001.