Characterization of VAMP-7–null platelets. (A) Transmission electron microscopy of platelets from wild-type (left) and VAMP-7^−/− mice (right). Scale bars represent 0.5 μm. (B) Immunoblot analysis of lysates derived from wild-type and VAMP-7^−/− platelets. (C) Immunoprecipitation of VAMP-7 from resting human platelets (Resting) and platelets stimulated with 5 μM SFLLRN (Activated). SNAP-23, syntaxin-11, and VAMP-7 were subsequently identified in immunoprecipitates using immunoblot analysis. WT, wild-type.