Loss of Gpx4 in hematopoietic cells induces anemia that is compensated by increased erythropoiesis. (A) Immunoblot analysis of Gpx4 in peripheral erythrocytes (TER119+) and bone marrow erythroid progenitors (CD71+/TER119+). Red blood cell counts (B), hemoglobin levels (C), and hematocrit (D) are decreased in Gpx4Δ mice, whereas the number of reticulocytes is increased (E). Data are mean ± SE; n ≥ 20. Elevated serum EPO levels (F) and enlarged spleens (G) in Gpx4Δ mice. Data are mean ± SE; n ≥ 10. (H-I) Immunohistochemistry staining of BrdU incorporation in the spleen and nuclear counterstain hematoxylin. Image acquisition was performed using a Zeiss Axio Imager M2 with a 20×/0.5 EC Plan Neofluar objective (magnification ×200). (J) Quantification of splenic BrdU+/TER119+ cells determined by flow cytometry. Data are mean ± SE; n ≥ 3. **P < .01, ***P < .001 by Student t test. BM, bone marrow; SE, standard error.
