Figure 1
Flow cytometric analysis of HEK293 cells expressing murine WT or R84A EPCR. (A) HEK293 cells were stably transfected with pcDNA3.1(−) vector only (left), or vectors containing the cDNA encoding murine WT (middle) or R84A (right) EPCR. EPCR expression was measured by incubating transfected HEK293 cells with FITC-IgG isotype control (filled histogram) or FITC-labeled goat polyclonal EPCR antibody (gray line). (B) Stably transfected HEK293 cells expressing WT or R84A mEPCR were incubated with murine Fl-APC (0-500 nM) in the presence of 3 mM CaCl2 and 0.6 mM MgCl2 for 15 minutes at room temperature and binding of Fl-labeled protein to cells expressing mEPCR was analyzed by flow cytometry. IgG, immunoglobulin G.
