EPCR expression in EPCR^R84A/R84A mice. (A) Western blot analysis of tissue lysates from heart, kidney, liver, lung, and spleen of WT and EPCR^R84A/R84A mice. The nitrocellulose membrane was stained with Ponceau S dye to confirm that similar amounts of β-actin protein were loaded in each lane. (B) Frozen sections of aorta from WT and EPCR^R84A/R84A mice were fixed with 4% paraformaldehyde and stained with a FITC-labeled rabbit polyclonal antibody specific for EPCR, and DAPI, a nuclear stain used to identify cells. Blots and images are representative of 3 mice per group. L, the lumen.