Platelets scavenge neutrophil elastase during IC-mediated inflammation. (A) Flow cytometry dot plots describing the identification of elastase-positive platelets in whole blood. Platelets were identified according to their light-scattering properties (left) and positivity for glycoprotein V (GPV) (middle). A subpopulation of platelets with highly positive elastase was then further identified using a fluorescent polyclonal antibody to neutrophil elastase (right). (B) Comparison of the proportion of elastase-high platelets among total circulating platelets before and 4 hours after inducing the cutaneous rpA reaction in wild-type and GPVI^−/− mice. n = 7-8 mice per group. (C) Mean elastase levels on circulating platelets before and 4 hours after inducing the cutaneous rpA reaction in wild-type and GPVI^−/− mice. Results are expressed relative to the mean fluorescence intensity for elastase measured on wild-type platelets before the induction of the rpA reaction. n = 7-8 mice per group. FSC-A, forward scatter area; SSC-A, side scatter area.