Collagen-independent thrombin generation is mediated by GPVI. (A) Peak of thrombin generation in PRP of controls and patients with a congenital deficiency of GPVI (n = 4). (B) Thrombin peak in human PRP stimulated with TF in the presence of a control Fab (50 µg/mL) and the anti-GPVI Fab 9O12 (50 µg/mL) (n = 13). (C) Dose-dependent effect of the Fab 9O12 on the thrombin peak (n = 3). (D) Thrombin peak in human PRP stimulated with thrombin (2 nmol/L) or kaolin (1 µg/mL) (n = 3). (E) Curves of thrombin generation in human PFP (dotted lines) and PRP (plain lines) stimulated with TF in the presence of a control Fab (black) and the Fab 9O12 (gray). (F) Effect of GPVI blockade on thrombin generation in human PFP and PRP with and without PGE1 (100 nmol/L) and apyrase (25 µg/mL) (n = 3). Mean ± SEM, *P < .05, ***P < .001, Mann-Whitney U test, Student t test.