The combined effect of CBD and an AKT inhibitor on t(8;21) leukemia cell growth and cell cycle. (A) Growth inhibition of AE9a cells, Kasumi-1 cells, and C-Kit⁺ HSPCs treated at different concentrations of CBD or AKT inhibitor, alone or together, at 72 hours. Values represent the mean ± SD for 3 separate experiments. (B and C) Cell cycle analysis on AE9a and Kasumi-1 cells treated with CBD alone, AKT inhibitor alone, or both together at 24 hours. BrdU was added during the last hour of incubation. Cells were then processed for double staining with anti-BrdU–allophycocyanin (APC) and 7 amino actinomycin D (7-AAD) and analyzed by flow cytometry.