Changes in body temperature induced by antibody 2.4G2 and 2.4G2 scFv-MSA. Mice were treated with 0.43 nmol (65 µg) 2.4G2 or equimolar amounts of 2.4G2 scFv-MSA or HSA. Cross-linked 2.4G2 scFv-MSA was prepared by mixing 0.43 nmol 2.4G2 scFv-MSA and half-molar (0.22 nmol) anti-His mAb for 30 minutes at room temperature. Body (rectal) temperature was measured 0.5, 1, 1.5, and 2 hours after treatment by a thermometer. n = 6–9, from 3 independent experiments.