Treatment with allogeneic CAR T cells following B6 → C3h.sw BMT resulted in early mortality. (A) Treatment protocol: mice received 106 E2a:PBX leukemia cells on day −5, followed by myeloablative radiation (1000 cGy) and a TCD BMT (3.5-4 × 106 cells) on day 0, followed by adoptive transfer of 106 CAR or mock T cells on day +2. (B) Leukemia-bearing B6 recipients received B6 (black) or C3h.sw (red) BM and T cells (CAR transduced in solid lines and mock T cells in dashed lines). Survival and weight change from baseline were monitored. (C) Allogeneic transplant was now with C3h.sw as recipients of B6 CAR (red, solid) or mock (red, dashed) T cells. Survival and weight change from baseline are shown. Survival graphs indicate pooled data from 2 replicate experiments.
