Akt phosphorylation levels increased in PDPN-stimulated megakaryocytes. Phosphorylation levels of Akt (A), p44/42 (B), and p38 (C) were examined by western blotting. The left images show representative bands for each target protein, and the results are quantified in the right graphs (n = 3 per group). The error bars reflect the SEMs. *P < .05. N.S., a nonsignificant difference.