Treatment with ruxolitinib lessens CpG-induced splenomegaly and cytopenias. (A) C57BL/6 (B6) mice were treated with PBS or CpG (50 μg) every other day as indicated (open/white arrow). Beginning on day 4, mice did or did not receive ruxolitinib (Ruxo) twice daily by oral gavage. On day 9, mice were euthanized and analyses performed. (B) Whole-spleen images from treatment groups. (C) Splenomegaly was quantified by measuring the ratio of spleen to body weight × 100. (D) Blood was analyzed for WBCs, RBCs, hemoglobin, platelets, neutrophils (NC), and lymphocytes (LC). Individual symbols each depict 1 mouse with horizontal lines representing the mean ± standard deviation (SD). Data are representative of 3 independent experiments. *P < .05.