Figure 4
Figure 4. Ruxolitinib treatment reduces LCMV-induced hypercytokinemias and ameliorates liver inflammation. (A) Serum cytokine levels were assessed on days 8 to 10. (B) H&E-stained liver sections demonstrate inflammatory infiltrates (dark purple clusters), indicated by arrows. Representative sections are shown at a magnification of ×20 (top panels) and following computer analysis of inflammatory area (bottom panels). (C) The number of inflammatory foci (left) and percent area occupied by inflammatory foci with respect to the HPF view (right) was determined as in Figure 2. Data shown as mean ± SD are representative of 4 experiments. (D) Daily body weight is depicted as a ratio of the current over initial body weight ×100 with statistical significance determined by 2-way ANOVA. Symbols in panel A represent individual mice in each treatment group; in panel C, they represent the number of, or area encompassed by, inflammatory foci (clusters containing >8 lymphocytes) per ×20 field of view, whereas in panel D they represent the mean daily weight of the animals in each cohort. (E) Overall survival is depicted and statistical significance was determined by the log-rank test. Data in panels D and E are representative of 4 and 2 independent experiments, respectively. *P < .05, **P < .001.

Ruxolitinib treatment reduces LCMV-induced hypercytokinemias and ameliorates liver inflammation. (A) Serum cytokine levels were assessed on days 8 to 10. (B) H&E-stained liver sections demonstrate inflammatory infiltrates (dark purple clusters), indicated by arrows. Representative sections are shown at a magnification of ×20 (top panels) and following computer analysis of inflammatory area (bottom panels). (C) The number of inflammatory foci (left) and percent area occupied by inflammatory foci with respect to the HPF view (right) was determined as in Figure 2. Data shown as mean ± SD are representative of 4 experiments. (D) Daily body weight is depicted as a ratio of the current over initial body weight ×100 with statistical significance determined by 2-way ANOVA. Symbols in panel A represent individual mice in each treatment group; in panel C, they represent the number of, or area encompassed by, inflammatory foci (clusters containing >8 lymphocytes) per ×20 field of view, whereas in panel D they represent the mean daily weight of the animals in each cohort. (E) Overall survival is depicted and statistical significance was determined by the log-rank test. Data in panels D and E are representative of 4 and 2 independent experiments, respectively. *P < .05, **P < .001.

Close Modal

or Create an Account

Close Modal
Close Modal