UCH-L1 is specifically induced in GCBs. (A) The expression of UCHL1 as reflected in RNA microarray data are shown for the indicated purified human B-cell subsets. Data extracted from GSE2350.13 (B) Formalin-fixed paraffin-embedded human reactive lymph node specimens were stained for UCH-L1 (brown). Bar: 500 μm (left), 200 μm (right). (C) Formalin-fixed paraffin-embedded human tonsil sections were stained with the indicated. Bar: 200 μm. (D) Quantitative real-time PCR for murine Uchl1 was performed on complementary DNA generated from GC or non-GCBs purified from wild-type mice (n = 3 each); *P < .05. (E) Extracts were prepared from the indicated purified B-cell subsets (n = 2 each), and samples were immunoblotted for the indicated proteins. Histone H2B is included as a loading control. Microscopy images were obtained with an Olympus AX70 microscope with a DP71 camera.
