Zbtb16^lu/lu HSCs shows cell cycle deregulation. HSCs from Zbtb16^lu/lu and WT mice were subjected to gene expression analysis. (A) Gene set enrichment analysis of Zbtb16^lu/lu LSKs compared with WT LSKs. (B) Quantitative polymerase chain reaction analysis of cell cycle–related gene expression in Zbtb16^lu/lu and WT LT-HSCs sorted from young mice. Results are presented as relative to messenger RNA (mRNA) expression level. (C) Distribution of the CD150⁺ HSPC (left) and MPP2 cell fractions in G0, G1, and S-G2/M phases. Bars represent average ± SEM. Data were collected over 3 independent experiments (n = 7). (D) Distribution of Zbtb16^lu/lu and WT CD150⁺ HSPCs in G1, S, and G2/M phases after BrdU injection. Bars represent average ± SEM. Data were collected over 2 independent experiments (n = 7). (E) Cell cycle analysis of Zbtb16^lu/lu and WT CD150⁺ HSPCs 6 days after myelosupression obtained by a single dose of 5-fluorouracil (5Fu; 150 mg/kg) (n = 5). Bars represent average ± SEM. Data were collected over 2 independent experiments (n = 5). (F) Analysis of apoptosis in Zbtb16^lu/lu and WT CD150⁺ HSPCs from 8- to 10-week-old mice (i) and from transplanted mice with 8- to 10-week-old BM (ii). Bars represent mean ± SEM (n = 5). *P < .05; **P < .01; ***P < .01. ns, not significant.