A combination of colocalization of TF binding and genome-wide interaction data identifies previously unknown hematopoietic enhancer elements. Significant interactions as identified by the CHiCAGO pipeline were loaded into the WashU browser as a custom track (interactions) along with the bigwig tracks of the previously published HPC-7 ChIP-Seq data.^17,18  Only interactions where both interacting fragments are within the genomic window are shown. For visualization purposes, the promoters used as “bait” in the CHi-C protocol are also shown (promoter fragments), the individual restriction enzyme fragments (HindIII restriction fragments) and a track showing RefSeq genes. All tracks shown are in mouse genome build mm9. (A) Hhex locus; shown for verification are the previously identified regulatory elements and the newly identified Hhex +59 kb enhancer. (B) Cebpα locus; shown for verification is the Cebpα +37 kb enhancer. (C) Transgenic analysis of E11.5 X-Gal (5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside)-stained whole-mount embryos and paraffin sections of dorsal aorta (DA) and fetal liver (FL). (i) Hhex +59 kb. (ii) Cebpα +37 kb.