DXM modulation enhanced CD4⁺ and CD8⁺ T cell suppression by cytokine-induced CD33⁺ cells. (A) The inhibitory effect of in vitro-generated MDSCs on CD4⁺ T-cell proliferation. The number of peaks resembles cell division processes in DXM-modulated and unmodulated groups. (B-C) The division index of lymphocyte proliferation using in vitro-generated MDSCs from 8 randomly selected patients at 1:2 (B) and 1:4 (C) ratios. (D) A representative scatter-gram of JC1 mitochondrial potential test for platelet apoptosis. JC1 is a mitochondrial membrane potential-sensitive carbocyanine probe. Monomeric green fluorescent JC1 is taken up by high-membrane potential mitochondria, where it reversibly forms red fluorescent aggregates with loss of mitochondrial membrane potential. (E) Apoptosis level of platelets (PLT) alone (2.714 ± 0.296), platelets cocultured with CD8⁺ T cells (CTL; 16.094 ± 2.676), platelets cocultured with MDSC-primed CD8⁺ T cells (9.992 ± 1.431), and platelets cocultured with DXM-MDSC-primed CD8⁺ T cells (6.206 ± 0.981), using in vitro-generated MDSCs from 10 randomly selected patients. Differences between each 2 groups were compared using the Mann-Whitney test. **P < .01.