α_IIb and β₃ expression is increased in Dicer1-deficient platelets. (A) RNA-seq reads from Dicer1^Pf4Δ/Pf4Δ or Dicer1^fl/fl platelets aligning to α_IIb (top) or β₃ (bottom) mRNAs. Refseq annotations are included underneath each tracing, where thick and thin lines represent exons and introns, respectively. In these depictions, reads are piled up at their alignment location, so that both the density and height of histogram peaks are indicative of relative abundance. RNA-seq was performed on a pool of 3 mice per group. (B-C) Real-time PCR expression analysis of α_IIb or β₃ mRNA in (B) platelets or (C) BM-derived MKs from Dicer1^Pf4Δ/Pf4Δ vs Dicer1^fl/fl mice. *P < .05; **P < .01; unpaired Student t test. n = 9 to 13 mice per group for (B) and n = 4 mice per group for (C). (D) Surface expression of α_IIb and β₃ protein. Platelets from Dicer1^Pf4Δ/Pf4Δ or Dicer1^fl/fl mice were isolated and stained in littermate pairs for flow cytometry analysis. The gMFI of surface staining of α_IIb and β₃ for each pair are shown. Light gray lines connect each matched littermate pair, and the horizontal lines represent the mean for each group (**P < .01; paired Student t test; n = 10 pairs).