miRNAs that are decreased in Dicer1-deficient platelets regulate α_IIb and β₃ expression through their 3′UTR. HEK293 cells were transfected with a reporter gene construct that contains the 3′-UTR sequence of α_IIb (A) or β₃ (B), located downstream of the Rluc open reading frame, and vectors encoding pre–mmu-miR-128, pre–mmu-miR-500, pre–mmu-miR-331, pre–mmu-miR-326, or a nonrelevant short hairpin control. Rluc and Firefly luciferase signals were measured and normalized to the nonrelevant hairpin control vector, which is represented by the dashed line (set at 1). Shown are mean ± SEM. Analysis of variance: P < .05; *P < .05 vs control; unpaired Student t test. n = 5 to 6 experiments performed in duplicate.