Analysis of signaling events in primary BCR-ABL1–expressing myeloid cells. (A) Representative immunoblot of primary Lin^–GFP⁺ cell extracts from WT or Gab2^−/− BM cells transduced with BCR-ABL1 p210MIGFP retrovirus; similar results were obtained in ≥6 independent biological replicates. (B) Statistical analysis of signaling events in A, pooled from ≥6 biological replicates (***P < .001, **P < .01, *P < .05, n.s., not significant; 2-sided Student t tests). (C) Representative immunoblot of primary Lin^–GFP⁺RFP⁺ cell extracts from Gab2^−/− BM cells cotransduced with p210-IRES-RFP and Gab2-IRES-GFP viruses, as indicated. Similar results were obtained in ≥5 biological replicates. (D) Statistical analysis of signaling events pooled from ≥5 biological replicates of the experiment shown in C (***P < .001, **P < .01, *P < .05, n.s., not significant; all vs Gab2WT cotransduced samples; 2-sided Student t tests).