Analysis of signaling events in primary BCR-ABL1–expressing B-lymphoid progenitors. (A) Representative immunoblot of primary CD127⁺GFP⁺ cell extracts from Gab2^+/+ (WT) or Gab2^−/− BM cells transduced with BCR-ABL1 p210MIGFP retrovirus; similar results were obtained in 3 independent biological replicates. (B) Statistical analysis of signaling events pooled from 3 biological replicates of the experiment shown in A (**P < .01, *P < .05; 1-sided Student t tests). (C) Representative immunoblot of primary CD127⁺GFP⁺RFP⁺ cell extracts from Gab2^−/− BM cells cotransduced with p210-IRES-RFP and Gab2-IRES-GFP viruses, as indicated. Similar results were obtained in 3 biological replicates. (D) Statistical analysis of signaling events pooled from 3 biological replicates of the experiment shown in C (**P < .01, *P < .05; P = .1 for p-STAT5 between Gab2ΔShp2 and Gab2WT; 1-sided Student t tests).