GAB2 deficiency affects transcription of downstream effectors. RNA-seq was performed on BCR-ABL1–transformed WT or Gab2^−/− pre-B cells. (A) Unsupervised clustering of samples based on expression of all genes. (B) Heat map showing differential gene expression (supervised analysis) in WT and Gab2^−/− cells. (C-E) mRNA levels of typical transcriptional targets downstream of the (C) ERK, (D) AKT, or (E) STAT5 pathway were altered (*FDR < 0.05, CuffDiff). (F-G) gene set enrichment analysis of Gene Ontology term enrichment in Gab2^−/− compared with WT cells. Gene sets in biological processes of (F) RNA processing and (G) DNA replication were significantly enriched in downregulated genes in Gab2^−/− cells. FDR < 0.01 for both (F) and (G).