Detection of CD41⁺P-selectin⁺ Tregs in peripheral blood of healthy volunteers, HIV-infected adults, and patients with ACS. Flow cytometry analyses of peripheral blood obtained from patients with ACS (n = 10), HIV-infected adults on stable combination antiretroviral therapy (n = 20), and healthy volunteers (HV; n = 17) for surface CD4, CD127, CD25, CD41, P-selectin, and intracellular FOXP3 expression. (A) Representative example of CD4⁺CD25^highCD127^low/−FOXP3⁺ Treg gating and their exposure of CD41 and P-selectin in peripheral blood of a healthy volunteer. (B) Percentage of CD41 and P-selectin double-positive Tregs in the peripheral blood of ACS patients, HIV-infected adults, and healthy volunteers.