Longer CDR3 sequences and increased representation of positively charged amino acids in switched memory B cells. (A) Amino acid length of CDR3 sequences in FACS-sorted naïve and switched memory B cells from HD (data pooled from 3 individuals), P1, and P2 analyzed by deep sequencing; ***P < .001 and ****P < .0001 (unpaired Student t test). (B) Distribution of number of positively charged amino acids (0, 1, 2, ≥3) per H-chain V region of sorted switched memory B-cell in HD (data pooled from 3 individuals), P1, and P2 analyzed by deep sequencing. In P2, FAS-competent (FAS^wt/c.3G>T) and FAS-deficient (FAS^{c.3G>T/c.3G>T}) memory cells are analyzed separately.