Morphologic analysis of organ tissues from PtenΔ/Δ; Nf1^+/− mice induced at PND8 and from control littermates at age 3 weeks (2 weeks post–poly[I:C]). Cytospins from BM show increased granulocytes and monocytes and substantial monocytic infiltration was found in the lungs, liver, and spleen of mice with PtenΔ/Δ; Nf1^+/−. The lungs show nodular monocytic infiltration (indicated by an arrow). Monocytic infiltration was observed in the sinuses of the liver (indicated by arrows). Monocytic infiltration involved both red and white pulps of the spleen, which is accompanied by decreased lymphoid tissues. Images were acquired using a Nikon Eclipse NV with a 100× lens (BM cytospins) and an Aperio CS2 scanner (HE and IHC). BM, bone marrow; HE, hematoxylin and eosin staining, IHC, immunohistochemistry staining with indicated specific antibodies.