FACS analysis of cell subpopulations from the BM, PB, and spleens of PtenΔ/Δ; Nf1^+/− mice induced at PND8 and of control littermates at age 3 to 4 weeks (2-3 weeks post–poly[I:C]). Mice with PtenΔ/Δ, regardless of Nf1 background, had significantly increased monocytes/macrophages and granulocytes (A-C) in BM, PB, and spleen, but elevated mature monocytes were seen in BM and PB from mice with PtenΔ/Δ; Nf1^+/− but not PtenΔ/Δ; Nf1^+/+ (C). B cells (D) and T cells (E) were both markedly decreased in PB and spleen from mice with PtenΔ/Δ, regardless of Nf1 background; macrophage infiltration was confirmed in spleens from mice with PtenΔ/Δ, regardless of Nf1 background (F). Data are presented as mean ± SD (n = 7-21 per group). *P < .05; **P < .01; ***P < .001. Additional supportive data are presented in supplemental Table 3 and supplemental Figure 4.